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Huabio Inc anti jun
Anti Jun, supplied by Huabio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Related Articles

Membrane:

Article Title: Targeting cell membrane sphingolipids: a novel ceramide analog CeY-B1 inhibits migration and invasion in cervical cancer cells
Article Snippet: Proteins were separated by SDS-PAGE (LABGIC, BL522A) and transferred to a PVDF membrane (Merck Millipore, IPVH00010). .. The membrane was incubated with primary antibodies against JNK (1:1000), phosphorylated JNK (P-JNK, 1:1500, Proteintech, 80024-1-RR), cytochrome C1 (Cyt c, 1:1000, UpingBio, YP-Ab-00366), BCL-2 (1:1000, UpingBio, YP-Ab-00322), BAX (1:1000, UpingBio, YP-Ab-00317), GAPDH (1:10,000, UpingBio, YP-Ab-03483), PI3 Kinase p110 Beta (1:1000, proteintech, 20584-1-AP), AKT (1:2000, proteintech, 10176-2-AP) and P-AKT (Ser473) (1:1000, proteintech, CL488-66444), p-c-Jun (1:1000, HUABIO, ET1608-4), c-Jun (1:1000, HUABIO, ET1608-3), β-Tubulin (1:1000, Cell Signaling, 2128 S). .. Detection was performed using ECL chemiluminescent substrate (Biosharp, BL520B).

Article Title: Targeting cell membrane sphingolipids: a novel ceramide analog CeY-B1 inhibits migration and invasion in cervical cancer cells.
Article Snippet: Proteins were separated by SDS-PAGE (LABGIC, BL522A) and transferred to PVDF membrane (Merck Millipore, IPVH00010). .. The membrane was incubated with primary antibodies against JNK (1:1000); phosphorylated JNK (P-JNK, 1:1500, Proteintech, 80024-1-RR); cytochrome C1 (Cyt c, 1:1000, UpingBio, YP-Ab-00366); BCL-2 (1:1000, UpingBio, YP-Ab-00322); BAX (1:1000, UpingBio, YP-Ab-00317); GAPDH (1:10,000, UpingBio, YP-Ab-03483); PI3 Kinase p110 Beta (1:1000, proteintech, 20584-1-AP); AKT (1:2000, proteintech, 10176-2-AP) and P-AKT (Ser473) (1:1000, proteintech, CL488-66444); p-c-Jun (1:1000, HUABIO, ET1608-4); c-Jun (1:1000, HUABIO, ET1608-3); β-Tubulin (1:1000, Cell Signaling, 2128S). .. Detection was performed using ECL chemiluminescent substrate (Biosharp, BL520B).

Incubation:

Article Title: Targeting cell membrane sphingolipids: a novel ceramide analog CeY-B1 inhibits migration and invasion in cervical cancer cells
Article Snippet: Proteins were separated by SDS-PAGE (LABGIC, BL522A) and transferred to a PVDF membrane (Merck Millipore, IPVH00010). .. The membrane was incubated with primary antibodies against JNK (1:1000), phosphorylated JNK (P-JNK, 1:1500, Proteintech, 80024-1-RR), cytochrome C1 (Cyt c, 1:1000, UpingBio, YP-Ab-00366), BCL-2 (1:1000, UpingBio, YP-Ab-00322), BAX (1:1000, UpingBio, YP-Ab-00317), GAPDH (1:10,000, UpingBio, YP-Ab-03483), PI3 Kinase p110 Beta (1:1000, proteintech, 20584-1-AP), AKT (1:2000, proteintech, 10176-2-AP) and P-AKT (Ser473) (1:1000, proteintech, CL488-66444), p-c-Jun (1:1000, HUABIO, ET1608-4), c-Jun (1:1000, HUABIO, ET1608-3), β-Tubulin (1:1000, Cell Signaling, 2128 S). .. Detection was performed using ECL chemiluminescent substrate (Biosharp, BL520B).

Article Title: M2 polarization of macrophage induced by IL-33 promotes mouse sciatic nerve regeneration.
Article Snippet: For hematoxylin staining, sections of muscles were stained with hematoxylin (Nanjing Jiancheng Bioengineering Institue) for 15 s, and mounted with neutral resin. .. For immunofluorescent staining, the sections of nerves were incubated with primary antibodies including anti-rabbit IL-33 (1:500, ab187060, Abcam), anti-rabbit GAP43 (1:500, 8945S, CST), anti-rabbit CD206 (1:500, ab3003621, Abcam), anti-rabbit CD86 (1:200 , ET1606-50, HuaBio), anti-rabbit IBA1 (1:500, ab178846, Abcam), anti-rabbit S100β (1:500, 66616–1- lg, Proteintech), anti-mouse Sox10 (1:200, 66786-1-Ig, Proteintech), anti-rabbit c-Jun (1:200 ET-1608-4, HuaBio), anti-rabbit Ki67 (1:200, HA71115, HuaBio), followed by incubated with secondary antibodies including donkey anti-mouse Alexa Fluor 555 (1:500, A-31570, Thermo Fisher), donkey anti-rabbit Alexa Fluor 488 (1:500, A-21206, .. AR TIC LE IN PR ES S ARTICLE IN PRESS 10 Thermo Fisher).

Article Title: M2 polarization of macrophage induced by IL-33 promotes mouse sciatic nerve regeneration
Article Snippet: For hematoxylin staining, sections of muscles were stained with hematoxylin (Nanjing Jiancheng Bioengineering Institue) for 15 s, and mounted with neutral resin. .. For immunofluorescent staining, the sections of nerves were incubated with primary antibodies including anti-rabbit IL-33 (1:500, ab187060, Abcam), anti-rabbit GAP43 (1:500, 8945S, CST), anti-rabbit CD206 (1:500, ab3003621, Abcam), anti-rabbit CD86 (1:200, ET1606-50, HuaBio), anti-rabbit IBA1 (1:500, ab178846, Abcam), anti-rabbit S100β (1:500, 66616-1-lg, Proteintech), anti-mouse Sox10 (1:200, 66786–1-Ig, Proteintech), anti-rabbit c-Jun (1:200 ET-1608–4, HuaBio), anti-rabbit Ki67 (1:200, HA71115, HuaBio), followed by incubated with secondary antibodies including donkey anti-mouse Alexa Fluor 555 (1:500, A-31570, Thermo Fisher), donkey anti-rabbit Alexa Fluor 488 (1:500, A-21206, Thermo Fisher). .. After mounting with anti-fade medium (Vector), immunofluorescent images were acquired using an EVOS M5000 fluorescence microscope (Thermo Fisher) with consistent exposure settings across samples.

Article Title: Integrating Network Pharmacology and Experimental Validation to Identifying Key Herbal Components and Targets for Liver Cancer
Article Snippet: .. The membranes were blocked and incubated overnight at 4°C with antibodies, including anti-β-Tubulin (1:10000, AB0039, Abways, China), anti-c-Jun (1:1000, ET1608-3, HUABIO, China), antiP-c-Jun (1:2000, ET1608-4, HUABIO), anti-c-Fos, and anti-Pc-Fos (1:500, ET1701-95, HUABIO). ..

Article Title: Erbin Confers Neuroprotection Against Cerebral Ischemia-Reperfusion Injury in Mice via MAPK Pathway Inhibition
Article Snippet: Protein extraction was performed, and 251 the total protein concentration was determined using a commercial bicinchoninic acid 252 assay (BCA) kit (Yeasen Biotechnology, China), followed by subjecting the samples to 253 8% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) or 4%-20% 254 gradient gel (ACE Biotechnology, China) followed by electro-transfer onto a 255 polyvinylidene fluoride (PVDF) membrane. .. 256 The membranes were incubated overnight at 4°C with the primary antibodies 257 against the following proteins: Erbin (1:1000, NBP2-56104, Novus, USA), ERK1/2 258 (1:500, ET1601-29, Huabio, China), p-ERK1/2 (1:1000, ET1610-13, Huabio, China), 259 JNK1/2/3 (1:500, ET1601-28, Huabio, China), p-JNK1/2/3 (1:500, ET1609-42, Huabio, 260 China), p38 (1:1000, ET1702-65, Huabio, China), p-p38 (1:1000, ER2001-52, Huabio, 261 China), β-tubulin (1:10000, ET1602-4, Huabio, China). ..

Article Title: Targeting cell membrane sphingolipids: a novel ceramide analog CeY-B1 inhibits migration and invasion in cervical cancer cells.
Article Snippet: Proteins were separated by SDS-PAGE (LABGIC, BL522A) and transferred to PVDF membrane (Merck Millipore, IPVH00010). .. The membrane was incubated with primary antibodies against JNK (1:1000); phosphorylated JNK (P-JNK, 1:1500, Proteintech, 80024-1-RR); cytochrome C1 (Cyt c, 1:1000, UpingBio, YP-Ab-00366); BCL-2 (1:1000, UpingBio, YP-Ab-00322); BAX (1:1000, UpingBio, YP-Ab-00317); GAPDH (1:10,000, UpingBio, YP-Ab-03483); PI3 Kinase p110 Beta (1:1000, proteintech, 20584-1-AP); AKT (1:2000, proteintech, 10176-2-AP) and P-AKT (Ser473) (1:1000, proteintech, CL488-66444); p-c-Jun (1:1000, HUABIO, ET1608-4); c-Jun (1:1000, HUABIO, ET1608-3); β-Tubulin (1:1000, Cell Signaling, 2128S). .. Detection was performed using ECL chemiluminescent substrate (Biosharp, BL520B).

Western Blot:

Article Title: Multi-pathway mechanisms of Liujunzi decoction in promoting glioma apoptosis and reversing drug resistance: network pharmacology and experimental validation
Article Snippet: CCK-8 kit was obtained from Sangon Biotech (Shanghai, China; Cat. No. E606335-0500), while the apoptosis detection kit (Cat. No. A211-01) was sourced from Vazyme (Jiangsu, China). .. The antibodies used in the Western blot were purchased from HUABIO (Hangzhou, China), with the following details: AKT (ET1609-51, 1:1,000), phosphorylated AKT (p-AKT, ET1607-73, 1:1,000), phosphorylated STAT3 (p-STAT3, ET1603-40, 1:1,000), phosphorylated c-Jun (p-c-Jun, HA722475, 1:1,000), c-Myc (HA722895, 1:1,000), HIF-1α (HA721997, 1:1,000), Cleaved caspase 3 (HA722367, 1:1,000). .. Antibody for GAPDH was purchased from Cell Signaling Technology (#5174, CST, 1:1,000).

Staining:

Article Title: M2 polarization of macrophage induced by IL-33 promotes mouse sciatic nerve regeneration.
Article Snippet: For hematoxylin staining, sections of muscles were stained with hematoxylin (Nanjing Jiancheng Bioengineering Institue) for 15 s, and mounted with neutral resin. .. For immunofluorescent staining, the sections of nerves were incubated with primary antibodies including anti-rabbit IL-33 (1:500, ab187060, Abcam), anti-rabbit GAP43 (1:500, 8945S, CST), anti-rabbit CD206 (1:500, ab3003621, Abcam), anti-rabbit CD86 (1:200 , ET1606-50, HuaBio), anti-rabbit IBA1 (1:500, ab178846, Abcam), anti-rabbit S100β (1:500, 66616–1- lg, Proteintech), anti-mouse Sox10 (1:200, 66786-1-Ig, Proteintech), anti-rabbit c-Jun (1:200 ET-1608-4, HuaBio), anti-rabbit Ki67 (1:200, HA71115, HuaBio), followed by incubated with secondary antibodies including donkey anti-mouse Alexa Fluor 555 (1:500, A-31570, Thermo Fisher), donkey anti-rabbit Alexa Fluor 488 (1:500, A-21206, .. AR TIC LE IN PR ES S ARTICLE IN PRESS 10 Thermo Fisher).

Article Title: M2 polarization of macrophage induced by IL-33 promotes mouse sciatic nerve regeneration
Article Snippet: For hematoxylin staining, sections of muscles were stained with hematoxylin (Nanjing Jiancheng Bioengineering Institue) for 15 s, and mounted with neutral resin. .. For immunofluorescent staining, the sections of nerves were incubated with primary antibodies including anti-rabbit IL-33 (1:500, ab187060, Abcam), anti-rabbit GAP43 (1:500, 8945S, CST), anti-rabbit CD206 (1:500, ab3003621, Abcam), anti-rabbit CD86 (1:200, ET1606-50, HuaBio), anti-rabbit IBA1 (1:500, ab178846, Abcam), anti-rabbit S100β (1:500, 66616-1-lg, Proteintech), anti-mouse Sox10 (1:200, 66786–1-Ig, Proteintech), anti-rabbit c-Jun (1:200 ET-1608–4, HuaBio), anti-rabbit Ki67 (1:200, HA71115, HuaBio), followed by incubated with secondary antibodies including donkey anti-mouse Alexa Fluor 555 (1:500, A-31570, Thermo Fisher), donkey anti-rabbit Alexa Fluor 488 (1:500, A-21206, Thermo Fisher). .. After mounting with anti-fade medium (Vector), immunofluorescent images were acquired using an EVOS M5000 fluorescence microscope (Thermo Fisher) with consistent exposure settings across samples.



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AP-1 (c-FOS and <t>c-JUN)</t> is a key regulator of PCI-34051 in the regulation of HASMC ferroptosis. (A) Volcano plot displaying the gene expression distribution and differentially expressed genes among the DMSO and PCI-34051 groups after treatment with CD. (B) Gene Ontology (GO) enrichment analysis showed that biological processes and cellular components were associated with these differentially expressed genes. (C, D) Western-blot analysis and quantification showing the protein levels of c-FOS and c-JUN in HASMCs after treatment with DMSO and PCI-34051 under the ferroptosis models induced by <t>CD.</t> <t>β-Actin</t> served as a loading control ( n = 4 per group). (E, F) Co-immunoprecipitation results showed that HDAC8 interacted with c-JUN in HASMCs. (G) Results of GST pull-down demonstrated a direct interaction between HDAC8 and c-JUN in HASMCs. Values are means ± SD; *** P < 0.001. BP, biological process. CC, cellular component. MF, molecular function.
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AP-1 (c-FOS and <t>c-JUN)</t> is a key regulator of PCI-34051 in the regulation of HASMC ferroptosis. (A) Volcano plot displaying the gene expression distribution and differentially expressed genes among the DMSO and PCI-34051 groups after treatment with CD. (B) Gene Ontology (GO) enrichment analysis showed that biological processes and cellular components were associated with these differentially expressed genes. (C, D) Western-blot analysis and quantification showing the protein levels of c-FOS and c-JUN in HASMCs after treatment with DMSO and PCI-34051 under the ferroptosis models induced by <t>CD.</t> <t>β-Actin</t> served as a loading control ( n = 4 per group). (E, F) Co-immunoprecipitation results showed that HDAC8 interacted with c-JUN in HASMCs. (G) Results of GST pull-down demonstrated a direct interaction between HDAC8 and c-JUN in HASMCs. Values are means ± SD; *** P < 0.001. BP, biological process. CC, cellular component. MF, molecular function.
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AP-1 (c-FOS and <t>c-JUN)</t> is a key regulator of PCI-34051 in the regulation of HASMC ferroptosis. (A) Volcano plot displaying the gene expression distribution and differentially expressed genes among the DMSO and PCI-34051 groups after treatment with CD. (B) Gene Ontology (GO) enrichment analysis showed that biological processes and cellular components were associated with these differentially expressed genes. (C, D) Western-blot analysis and quantification showing the protein levels of c-FOS and c-JUN in HASMCs after treatment with DMSO and PCI-34051 under the ferroptosis models induced by <t>CD.</t> <t>β-Actin</t> served as a loading control ( n = 4 per group). (E, F) Co-immunoprecipitation results showed that HDAC8 interacted with c-JUN in HASMCs. (G) Results of GST pull-down demonstrated a direct interaction between HDAC8 and c-JUN in HASMCs. Values are means ± SD; *** P < 0.001. BP, biological process. CC, cellular component. MF, molecular function.
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Image Search Results


AP-1 (c-FOS and c-JUN) is a key regulator of PCI-34051 in the regulation of HASMC ferroptosis. (A) Volcano plot displaying the gene expression distribution and differentially expressed genes among the DMSO and PCI-34051 groups after treatment with CD. (B) Gene Ontology (GO) enrichment analysis showed that biological processes and cellular components were associated with these differentially expressed genes. (C, D) Western-blot analysis and quantification showing the protein levels of c-FOS and c-JUN in HASMCs after treatment with DMSO and PCI-34051 under the ferroptosis models induced by CD. β-Actin served as a loading control ( n = 4 per group). (E, F) Co-immunoprecipitation results showed that HDAC8 interacted with c-JUN in HASMCs. (G) Results of GST pull-down demonstrated a direct interaction between HDAC8 and c-JUN in HASMCs. Values are means ± SD; *** P < 0.001. BP, biological process. CC, cellular component. MF, molecular function.

Journal: Life Medicine

Article Title: HDAC8-selective inhibitor PCI-34051 protects against aortic dissection by attenuating ferroptosis of vascular smooth muscle cells

doi: 10.1093/lifemedi/lnag013

Figure Lengend Snippet: AP-1 (c-FOS and c-JUN) is a key regulator of PCI-34051 in the regulation of HASMC ferroptosis. (A) Volcano plot displaying the gene expression distribution and differentially expressed genes among the DMSO and PCI-34051 groups after treatment with CD. (B) Gene Ontology (GO) enrichment analysis showed that biological processes and cellular components were associated with these differentially expressed genes. (C, D) Western-blot analysis and quantification showing the protein levels of c-FOS and c-JUN in HASMCs after treatment with DMSO and PCI-34051 under the ferroptosis models induced by CD. β-Actin served as a loading control ( n = 4 per group). (E, F) Co-immunoprecipitation results showed that HDAC8 interacted with c-JUN in HASMCs. (G) Results of GST pull-down demonstrated a direct interaction between HDAC8 and c-JUN in HASMCs. Values are means ± SD; *** P < 0.001. BP, biological process. CC, cellular component. MF, molecular function.

Article Snippet: The antibodies applied in this study were: β-Actin (AC026, ABclonal), FSP1 (20886-1-AP, Proteintech), GPX4 (ab125066, Abcam), c-JUN (T55290F, ABmart), c-FOS (T56596F, ABmart), Flag (F1804, Sigma-Aldrich), HDAC8 (17548-1-AP, Proteintech), SLC7A11 (26864-1-AP, Proteintech), 4-HNE (MAB3249-SP, Bio-techne), CD86 (13395-1-AP, Proteintech), α-SMA (ab7817, Abcam), α-SMA (GTX100034, Genetex), GST (AE001, Abclonal).

Techniques: Gene Expression, Western Blot, Control, Immunoprecipitation

AP-1 (c-FOS and c-JUN) eliminated the effects of PCI-34051 on HASMC ferroptosis. All HASMCs were infected with lenti-Flag and lenti-c-FOS + lenti-c-JUN, and then these HASMCs were used for subsequent experiments. (A, B) The protein level of AP-1 (c-JUN and c-FOS) was detected by Western blot in HASMCs infected with lenti-Flag and lenti-c-JUN + lenti-c-FOS ( n = 4 per group). (C, D) The CCK8 assay showing the relative viability of HASMCs treated with DMSO and PCI-34051 after CD (C) and IKE (D) stimulation for the indicated time ( n = 5 per group). (E, F) Flow cytometry with propidium iodide (PI) staining displaying the percentage of PI-positive cells of HASMCs after treatment as described above ( n = 4 per group). (G, H) The LDH assay indicating the relative cell damage rate of HASMCs treated with described above ( n = 5 per group). (I–L) The ratio of oxidized BODIPY-C11/non-oxidized BODIPY-C11 fluorescence revealing the level of ROS of HASMCs treated with described above ( n = 4 per group). (M–P). 4-HNE immunofluorescence staining and quantitative analysis exhibiting the content of 4-HNE in HASMCs after treatment as described above ( n = 4 per group). (Q–T) Western-blot analysis and quantification performed to assess the protein levels of GPX4, FSP1, and SLC7A11 in AP-1 overexpressed HASMCs after DMSO and PCI-34051 treatment along with CD or IKE induction. β-Actin served as a loading control ( n = 4 per group). Values are means ± SD; *** P < 0.001, ** P < 0.01, * P < 0.05.

Journal: Life Medicine

Article Title: HDAC8-selective inhibitor PCI-34051 protects against aortic dissection by attenuating ferroptosis of vascular smooth muscle cells

doi: 10.1093/lifemedi/lnag013

Figure Lengend Snippet: AP-1 (c-FOS and c-JUN) eliminated the effects of PCI-34051 on HASMC ferroptosis. All HASMCs were infected with lenti-Flag and lenti-c-FOS + lenti-c-JUN, and then these HASMCs were used for subsequent experiments. (A, B) The protein level of AP-1 (c-JUN and c-FOS) was detected by Western blot in HASMCs infected with lenti-Flag and lenti-c-JUN + lenti-c-FOS ( n = 4 per group). (C, D) The CCK8 assay showing the relative viability of HASMCs treated with DMSO and PCI-34051 after CD (C) and IKE (D) stimulation for the indicated time ( n = 5 per group). (E, F) Flow cytometry with propidium iodide (PI) staining displaying the percentage of PI-positive cells of HASMCs after treatment as described above ( n = 4 per group). (G, H) The LDH assay indicating the relative cell damage rate of HASMCs treated with described above ( n = 5 per group). (I–L) The ratio of oxidized BODIPY-C11/non-oxidized BODIPY-C11 fluorescence revealing the level of ROS of HASMCs treated with described above ( n = 4 per group). (M–P). 4-HNE immunofluorescence staining and quantitative analysis exhibiting the content of 4-HNE in HASMCs after treatment as described above ( n = 4 per group). (Q–T) Western-blot analysis and quantification performed to assess the protein levels of GPX4, FSP1, and SLC7A11 in AP-1 overexpressed HASMCs after DMSO and PCI-34051 treatment along with CD or IKE induction. β-Actin served as a loading control ( n = 4 per group). Values are means ± SD; *** P < 0.001, ** P < 0.01, * P < 0.05.

Article Snippet: The antibodies applied in this study were: β-Actin (AC026, ABclonal), FSP1 (20886-1-AP, Proteintech), GPX4 (ab125066, Abcam), c-JUN (T55290F, ABmart), c-FOS (T56596F, ABmart), Flag (F1804, Sigma-Aldrich), HDAC8 (17548-1-AP, Proteintech), SLC7A11 (26864-1-AP, Proteintech), 4-HNE (MAB3249-SP, Bio-techne), CD86 (13395-1-AP, Proteintech), α-SMA (ab7817, Abcam), α-SMA (GTX100034, Genetex), GST (AE001, Abclonal).

Techniques: Infection, Western Blot, CCK-8 Assay, Flow Cytometry, Staining, Lactate Dehydrogenase Assay, Fluorescence, Immunofluorescence, Control

The working model of PCI-34051 regulating HASMC ferroptosis and aortic dissection in mice. PCI-34051 inhibits the ferroptosis of HASMC by affecting the interaction between HDAC8 and AP-1 (c-FOS and c-JUN) and further suppresses the occurrence and development of BAPN-induced aortic dissection in mice. (This figure is created with Biorender).

Journal: Life Medicine

Article Title: HDAC8-selective inhibitor PCI-34051 protects against aortic dissection by attenuating ferroptosis of vascular smooth muscle cells

doi: 10.1093/lifemedi/lnag013

Figure Lengend Snippet: The working model of PCI-34051 regulating HASMC ferroptosis and aortic dissection in mice. PCI-34051 inhibits the ferroptosis of HASMC by affecting the interaction between HDAC8 and AP-1 (c-FOS and c-JUN) and further suppresses the occurrence and development of BAPN-induced aortic dissection in mice. (This figure is created with Biorender).

Article Snippet: The antibodies applied in this study were: β-Actin (AC026, ABclonal), FSP1 (20886-1-AP, Proteintech), GPX4 (ab125066, Abcam), c-JUN (T55290F, ABmart), c-FOS (T56596F, ABmart), Flag (F1804, Sigma-Aldrich), HDAC8 (17548-1-AP, Proteintech), SLC7A11 (26864-1-AP, Proteintech), 4-HNE (MAB3249-SP, Bio-techne), CD86 (13395-1-AP, Proteintech), α-SMA (ab7817, Abcam), α-SMA (GTX100034, Genetex), GST (AE001, Abclonal).

Techniques: Dissection