anti jun (Huabio Inc)
Structured Review
Anti Jun, supplied by Huabio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/jun/anti+c+jun+rabbit/pm42288534-149-22-38
Average 86 stars, based on 1 article reviews
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Membrane:Article Title: Targeting cell membrane sphingolipids: a novel ceramide analog CeY-B1 inhibits migration and invasion in cervical cancer cells Article Snippet: Proteins were separated by SDS-PAGE (LABGIC, BL522A) and transferred to a PVDF membrane (Merck Millipore, IPVH00010). .. The membrane was incubated with primary antibodies against JNK (1:1000), phosphorylated JNK (P-JNK, 1:1500, Proteintech, 80024-1-RR), cytochrome C1 (Cyt c, 1:1000, UpingBio, YP-Ab-00366), BCL-2 (1:1000, UpingBio, YP-Ab-00322), BAX (1:1000, UpingBio, YP-Ab-00317), GAPDH (1:10,000, UpingBio, YP-Ab-03483), PI3 Kinase p110 Beta (1:1000, proteintech, 20584-1-AP), AKT (1:2000, proteintech, 10176-2-AP) and P-AKT (Ser473) (1:1000, proteintech, CL488-66444), Article Title: Targeting cell membrane sphingolipids: a novel ceramide analog CeY-B1 inhibits migration and invasion in cervical cancer cells. Article Snippet: Proteins were separated by SDS-PAGE (LABGIC, BL522A) and transferred to PVDF membrane (Merck Millipore, IPVH00010). .. The membrane was incubated with primary antibodies against JNK (1:1000); phosphorylated JNK (P-JNK, 1:1500, Proteintech, 80024-1-RR); cytochrome C1 (Cyt c, 1:1000, UpingBio, YP-Ab-00366); BCL-2 (1:1000, UpingBio, YP-Ab-00322); BAX (1:1000, UpingBio, YP-Ab-00317); GAPDH (1:10,000, UpingBio, YP-Ab-03483); PI3 Kinase p110 Beta (1:1000, proteintech, 20584-1-AP); AKT (1:2000, proteintech, 10176-2-AP) and P-AKT (Ser473) (1:1000, proteintech, CL488-66444); Incubation:Article Title: Targeting cell membrane sphingolipids: a novel ceramide analog CeY-B1 inhibits migration and invasion in cervical cancer cells Article Snippet: Proteins were separated by SDS-PAGE (LABGIC, BL522A) and transferred to a PVDF membrane (Merck Millipore, IPVH00010). .. The membrane was incubated with primary antibodies against JNK (1:1000), phosphorylated JNK (P-JNK, 1:1500, Proteintech, 80024-1-RR), cytochrome C1 (Cyt c, 1:1000, UpingBio, YP-Ab-00366), BCL-2 (1:1000, UpingBio, YP-Ab-00322), BAX (1:1000, UpingBio, YP-Ab-00317), GAPDH (1:10,000, UpingBio, YP-Ab-03483), PI3 Kinase p110 Beta (1:1000, proteintech, 20584-1-AP), AKT (1:2000, proteintech, 10176-2-AP) and P-AKT (Ser473) (1:1000, proteintech, CL488-66444), Article Title: M2 polarization of macrophage induced by IL-33 promotes mouse sciatic nerve regeneration. Article Snippet: For hematoxylin staining, sections of muscles were stained with hematoxylin (Nanjing Jiancheng Bioengineering Institue) for 15 s, and mounted with neutral resin. .. For immunofluorescent staining, the sections of nerves were incubated with primary antibodies including anti-rabbit IL-33 (1:500, ab187060, Abcam), anti-rabbit GAP43 (1:500, 8945S, CST), anti-rabbit CD206 (1:500, ab3003621, Abcam), anti-rabbit CD86 (1:200 , ET1606-50, HuaBio), anti-rabbit IBA1 (1:500, ab178846, Abcam), anti-rabbit S100β (1:500, 66616–1- lg, Proteintech), anti-mouse Sox10 (1:200, 66786-1-Ig, Proteintech), Article Title: M2 polarization of macrophage induced by IL-33 promotes mouse sciatic nerve regeneration Article Snippet: For hematoxylin staining, sections of muscles were stained with hematoxylin (Nanjing Jiancheng Bioengineering Institue) for 15 s, and mounted with neutral resin. .. For immunofluorescent staining, the sections of nerves were incubated with primary antibodies including anti-rabbit IL-33 (1:500, ab187060, Abcam), anti-rabbit GAP43 (1:500, 8945S, CST), anti-rabbit CD206 (1:500, ab3003621, Abcam), anti-rabbit CD86 (1:200, ET1606-50, HuaBio), anti-rabbit IBA1 (1:500, ab178846, Abcam), anti-rabbit S100β (1:500, 66616-1-lg, Proteintech), anti-mouse Sox10 (1:200, 66786–1-Ig, Proteintech), Article Title: Integrating Network Pharmacology and Experimental Validation to Identifying Key Herbal Components and Targets for Liver Cancer Article Snippet: .. The membranes were blocked and incubated overnight at 4°C with antibodies, including anti-β-Tubulin (1:10000, AB0039, Abways, China), Article Title: Erbin Confers Neuroprotection Against Cerebral Ischemia-Reperfusion Injury in Mice via MAPK Pathway Inhibition Article Snippet: Protein extraction was performed, and 251 the total protein concentration was determined using a commercial bicinchoninic acid 252 assay (BCA) kit (Yeasen Biotechnology, China), followed by subjecting the samples to 253 8% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) or 4%-20% 254 gradient gel (ACE Biotechnology, China) followed by electro-transfer onto a 255 polyvinylidene fluoride (PVDF) membrane. .. 256 The membranes were incubated overnight at 4°C with the primary antibodies 257 against the following proteins: Erbin (1:1000, NBP2-56104, Novus, USA), ERK1/2 258 (1:500, ET1601-29, Huabio, China), p-ERK1/2 (1:1000, ET1610-13, Huabio, China), 259 JNK1/2/3 (1:500, ET1601-28, Huabio, China), Article Title: Targeting cell membrane sphingolipids: a novel ceramide analog CeY-B1 inhibits migration and invasion in cervical cancer cells. Article Snippet: Proteins were separated by SDS-PAGE (LABGIC, BL522A) and transferred to PVDF membrane (Merck Millipore, IPVH00010). .. The membrane was incubated with primary antibodies against JNK (1:1000); phosphorylated JNK (P-JNK, 1:1500, Proteintech, 80024-1-RR); cytochrome C1 (Cyt c, 1:1000, UpingBio, YP-Ab-00366); BCL-2 (1:1000, UpingBio, YP-Ab-00322); BAX (1:1000, UpingBio, YP-Ab-00317); GAPDH (1:10,000, UpingBio, YP-Ab-03483); PI3 Kinase p110 Beta (1:1000, proteintech, 20584-1-AP); AKT (1:2000, proteintech, 10176-2-AP) and P-AKT (Ser473) (1:1000, proteintech, CL488-66444); Western Blot:Article Title: Multi-pathway mechanisms of Liujunzi decoction in promoting glioma apoptosis and reversing drug resistance: network pharmacology and experimental validation Article Snippet: CCK-8 kit was obtained from Sangon Biotech (Shanghai, China; Cat. No. E606335-0500), while the apoptosis detection kit (Cat. No. A211-01) was sourced from Vazyme (Jiangsu, China). .. The antibodies used in the Western blot were purchased from HUABIO (Hangzhou, China), with the following details: AKT (ET1609-51, 1:1,000), phosphorylated AKT (p-AKT, ET1607-73, 1:1,000), phosphorylated STAT3 (p-STAT3, ET1603-40, 1:1,000), Staining:Article Title: M2 polarization of macrophage induced by IL-33 promotes mouse sciatic nerve regeneration. Article Snippet: For hematoxylin staining, sections of muscles were stained with hematoxylin (Nanjing Jiancheng Bioengineering Institue) for 15 s, and mounted with neutral resin. .. For immunofluorescent staining, the sections of nerves were incubated with primary antibodies including anti-rabbit IL-33 (1:500, ab187060, Abcam), anti-rabbit GAP43 (1:500, 8945S, CST), anti-rabbit CD206 (1:500, ab3003621, Abcam), anti-rabbit CD86 (1:200 , ET1606-50, HuaBio), anti-rabbit IBA1 (1:500, ab178846, Abcam), anti-rabbit S100β (1:500, 66616–1- lg, Proteintech), anti-mouse Sox10 (1:200, 66786-1-Ig, Proteintech), Article Title: M2 polarization of macrophage induced by IL-33 promotes mouse sciatic nerve regeneration Article Snippet: For hematoxylin staining, sections of muscles were stained with hematoxylin (Nanjing Jiancheng Bioengineering Institue) for 15 s, and mounted with neutral resin. .. For immunofluorescent staining, the sections of nerves were incubated with primary antibodies including anti-rabbit IL-33 (1:500, ab187060, Abcam), anti-rabbit GAP43 (1:500, 8945S, CST), anti-rabbit CD206 (1:500, ab3003621, Abcam), anti-rabbit CD86 (1:200, ET1606-50, HuaBio), anti-rabbit IBA1 (1:500, ab178846, Abcam), anti-rabbit S100β (1:500, 66616-1-lg, Proteintech), anti-mouse Sox10 (1:200, 66786–1-Ig, Proteintech), |
